JOURNAL OF EVIDENCE BASED MEDICINE AND HEALTHCARE

Table of Contents

2019 Month : November Volume : 6 Issue : 46 Page : 2931-2934

Staging of Cytological Smears in HIV Lymphadenitis and Correlation with Histopathology.

Hemalatha A. N.1

Corresponding Author:
Dr. Hemalatha A. N,
8th Cross, RMV Extension,
1st Stage, Sadashiva Nagar,
Bangalore- 560080, Karnataka.
E-mail: drhmlthan@yahoo.co.in
DOI: 10.18410/jebmh/2019/611

ABSTRACT
BACKGROUND
Lymph nodes from HIV positive patients with progressive generalized lymphadenopathy undergo morphological changes associated with progression of disease. These changes can be categorized cytologically with correlation of histopathological findings. FNA cytology although unable to differentiate between the three histological stages, can establish in most instances a reactive lymphoid process. Lymphadenitis is a common finding which includes numerous infectious and noninfectious conditions. In this study I have included only patients who are confirmed and diagnosed as HIV positive, and presented with generalized lymphadenopathy.

METHODS
FNAC was performed smears were air dried, few preserved in 90% methanol simultaneously biopsy was done on these patients for histopathological examination. Smears are stained by pap and Leishman stains.

RESULTS
One hundred HIV positive patients were studied who presented with generalized lymphadenopathy. Cases of Reactive Lymphadenitis, which were diagnosed on Fine Needle aspiration, were studied and correlated with histopathological sections in following stages-
Type I pattern - Follicular hyperplasia with or without paracortical hyperplasia.
Type II pattern - Diffuse lymphoid hyperplasia with loss of Germinal centers.
Type III pattern - Marked lymphocytic depletion.

CONCLUSIONS
Patterns 1 & 2 are associated with persistent generalised lymphadenopathy type 11 is rarer. Hence one should be very cautious to study this staging, because it mimics various forms of lymphoma on FNA smears. Immunocytochemistry is helpful to confirm benign nature of the process by demonstrating polyclonality.

KEYWORDS
HIV, Lymph node, reactive process