Varun Kumar1, Randhir Kumar2, Chandan Kumar Poddar3, Ram Nagina Sinha4, Suman Kumar5, S. K. Shahi6, R. N. Pappu7, M. N. Singh8
BACKGROUND Surgical site infection (SSI) is one of the most common postoperative complications and causes signi?cant postoperative morbidity and mortality. We wanted to determine the incidence of surgical site infections in clean, clean contaminated and contaminated surgeries in our setting along with the factors related to patient and surgical procedures influencing the rate of postoperative surgical wound infections and determine bacteriological profile with antimicrobial susceptibility patterns of the isolates. METHODS 260 clinical samples received from the Department of Orthopaedic Surgery, in the Department of Microbiology at Lord Buddha Koshi Medical College and Hospital, Saharsa, Bihar were studied. The study period was from September 2018 to April 2019. A total of 260 Staphylococcus aureus isolated was identified by standard biochemical methods. Antibiotic susceptibility testing was performed in our Department by Kirby Bauer Disc Diffusion method. Methicillin resistance was detected in the Microbiology Department by using cefoxitin (30 µg) disc diffusion method as per CLSI guidelines 2016. RESULTS Out of 260 S. aureus isolates of the MRSA 94 (36.2%) were derived from pus samples 39 (34.33%), the S. aureus isolates derived from wound samples were MRSA 17 (52.38%), the S. aureus isolates derived from blood samples were MRSA 21 (31.78%), the S. aureus isolates derived from miscellaneous samples were MRSA 13 (41.67%) and the S. aureus isolates derived from urine samples were MRSA 5 (36.36%). CONCLUSIONS Surgical site infections are one of the most common hospital acquired infections and are an important cause of morbidity and mortality. Depending on the site of wound infection and clinical symptoms, the role of microbiology laboratory is to determine the clinically significant isolates, perform antimicrobial susceptibility testing, and subsequently provide guidance on the most appropriate treatment. This will help in successful wound management and will also assist in the control of antibiotic usage and hence curtail the spread of antibiotic-resistant bacteria.
