Aim: To evaluate the effect of low-frequency ultrasound with variable parameters and environments on biological activity of fucoidan within determination of human plasma coagulation values.
Methods: The study was performed using fucoidan derived from the tangle Fucus vesiculosus. Fucoidan was processed with a high-intensity low-frequency ultrasound in water environment with and without administration of surface-active substances. The effectiveness and change in biological activity of the polysaccharide was evaluated using clotting tests: Prothrombin Test (PT test), partial thromboplastin test (APTT test), and Thrombin Test (TT test).
Results: During this study, it was determined that fucoidan can extend the clotting time of human plasma in vitro both before and after ultrasound processing. Higher values were detected for fucoidan processed with the administration of surface-active substances. Following sonochemical processing, decreased fucoidan activity was detected by TT and APTT tests compared to the original polysaccharide. Increased ultrasound intensity leads to retained biopolymer activity in the APTT test. During determination of methods of coagulation process inhibition, the effect of processed fucoidan on the external and internal ways of blood clotting was confirmed. As a result of the study, the most favorable conditions for hydroacoustic processing aimed at maintaining the biological activity of fucoidan were determined.
Conclusion: Based on the obtained data, we can suggest that using fucoidan after sonochemical processing in the development of thrombolytic therapy products can be advantageous.
