Apoorva B.1, Sneha Mohan2, Mukesh Manjhi3, Dalip Kakru4
BACKGROUND
Pseudomonas aeruginosa is the most common gram-negative bacterium
associated with nosocomial infections. Active observation of changes in antibiotic
resistance of Pseudomonas aeruginosa is necessary for the selection of
appropriate antimicrobial agent for empirical therapy. This study was conducted
to determine the antibiotic susceptibility pattern of Pseudomonas aeruginosa
isolated from various clinical samples collected from patients admitted in critical
and non-critical areas.
METHODS
Pseudomonas aeruginosa isolates obtained from various samples in critical and
non-critical areas during one-year period were included in the study. The isolates
were identified using standard laboratory procedures, and the susceptibility was
checked using the Kirby-Bauer disk-diffusion assay according to Clinical and
Laboratory Standard Institute (CLSI) guidelines-2019.
RESULTS
During one-year period, 224 Pseudomonas aeruginosa isolates were isolated from
patients admitted to various units, out of which 143 (63.8 %) were from noncritical
areas and 81 (36.1 %) were from critical areas. Highest isolation from noncritical
area was observed from pus sample 49 (34.26 %) followed by sputum and
urine samples 46 (32.16 %) and 27 (16.78 %) respectively. Pseudomonas
aeruginosa isolated from critical areas were mainly from endotracheal aspirates 36
(44.4 %) and all were multidrug resistant (MDR) (36.3 %).
CONCLUSIONS
The present study helps in understanding the emergence of MDR strains in
intensive care units (ICUs). Thus, regular surveillance of antibiotic susceptibility
pattern is important for reducing the healthcare associated infection (HAI) rates
and antimicrobial resistance.
