Ramanand Kumar Pappu1 , Rohit Kumar2 , Chandan Kumar Poddar3 , Maheshwar Narayan Singh4
BACKGROUND Surgical site infections are the most common hospital acquired infections and are an important cause of morbidity and mortality. The objective of this study is to evaluate the causative microorganisms and antimicrobial sensitivity pattern in surgical site infections. METHODS A total of 300 various clinical samples received in Microbiology Department, Lord Buddha Koshi Medical College, Saharsa (Northern Bihar and Associated Hospital) from January 2019 to January 2020 were studied. In a total of 110 samples, Staphylococcus aureus was isolated, detected by standard biochemical methods. Antibiotic susceptibility testing was performing by Kirby Bauer Disc Diffusion method. Methicillin resistance was detected using cefoxitin (30 µg) disc diffusion method as per CLSI guidelines 2016. RESULTS Out of the 300 bacteria which were isolated, 160 were gram positive cocci (53.46%) and 140 were gram negative bacilli (46.54%). The most common pathogen was Staphylococcus aureus 109 (36.26%). The other organisms were Escherichia, Pseudomonas, Klebsiella, Citrobacter, Proteus, and Enterococcus. The Antimicrobial profile of 109 Staphylococcus aureus isolates was as follows- among MRSA, 100% were sensitive to linezolid and vancomycin, with moderate sensitivity (72.34%) to cefuroxime, gentamicin and least sensitivity to (23.91%) doxycycline, (21.95%) ciprofloxacin. CONCLUSIONS The incidence of surgical site infection differs widely between surgical procedures, hospitals, patients, and between surgeons. Isolation of MRSA patients and carriers in the hospitals, regular observation, and monitoring of antibiotic susceptibility pattern of the hospital and community of that region frequently and formulation of antibiotic policy may help in reducing the treatment failures.
