Atit Dineshchandra Shah1, Urvashi Natubhai Limbachia2, Bhavin K. Prajapati3, Lata Patel4, Dharati Tusharbhai Shah5, Jayshri D. Pethani6, Nasiruddin M. Shaikh7, Hiral Shah8
BACKGROUND
Non fermenting gram-negative bacilli (NFGNB) are a group of heterogenous,
aerobic and non-sporing saprophytic bacteria, found as commensals in humans
and other animals primarily causing opportunistic healthcare-associated infections.
They are innately resistant to many antibiotics and are known to acquire resistance
by various mechanisms. They pose a particular difficulty for the healthcare
community because multidrug resistance is common and increasing among them
and a number of strains have now been identified that exhibit pan drug resistance.
This study was conducted to isolate and identify various non-fermenter gram
negative bacilli (NFGNB), to study their antibiotic sensitivity pattern and their
clinical significance from various clinical samples.
METHODS
A study was undertaken from March 2019 to February 2020 to isolate NFGNB from
various clinical samples received for culture and sensitivity in the department of
microbiology in a tertiary care hospital, Ahmedabad. Non lactose fermenting
colonies on MacConkey agar plates were further processed by Vitek 2 to identify
them and to study their antimicrobial susceptibility testing (AST).
RESULTS
A total of 2010 NFGNB were isolated from various clinical samples and their AST
was evaluated by Vitek 2. Pseudomonas aeruginosa (52.7 %) and Acinetobacter
baumannii (36.5 %) were the most common NFGNB isolated. Carbapenem
resistance was 93 % for Acinetobacter species and 61 % for Pseudomonas species.
CONCLUSIONS
Accurate and rapid identification and antimicrobial susceptibility testing of NFGNB
help in early initiation of appropriate antimicrobial therapy and proper
management of patients thereby help in reducing emergence of MDR strains of
NFGNB, mortality and overall hospital stay.
